Fertility / Procedures / Slow freezing

Slow freezing for a high rate of viable embryos

Mastering the slow cooling rates and controlling the concentration of cryoprotectants is critical for initiation of ice nucleation and successful freezing.

Slow freezing
  • G-FreezeKit™/G-ThawKit™ Blast 282 455
  • FREEZE-KIT 1™/THAW-KIT 1™ 603 455

  • G-FreezeKit™/G-ThawKit™ Blast
    These solutions are part of the G-series™ and designed to support the survival of blastocysts during freezing. The freezing solutions contain glycerol and sucrose as cryoprotectants.

     

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  • FREEZE-KIT 1™/THAW-KIT 1™
    FREEZE-KIT1™/THAW-KIT 1™ contains freezing solutions for cryopreservation of cleavage-stage embryos. The phosphate buffer is supplemented with propanediol and sucrose. The solutions contain pharmaceutical grade Penicillin-G and are ready-to-use after pre-equilibration at +20 ± 5ºC.

     

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  • Enhance cryo-survivability with Hyaluronan
    Hyaluronan is the major glucosaminoglycan present in the follicular, oviductal and uterine fluids. It has been found to significantly improve embryo cryo-survivability. (Yakin K et al. Oral presentation. ESHRE 2004). G-FreezeKit Blast and G-ThawKit Blast are designed for freezing blastocysts and contains hyaluronan for increased cryosurvability.
  • Simplify handling and ensure optimal heat transfer
    Each well in the 5-well dish is surrounded by either air or medium and the dish bottom is flat, resulting in a homogenous temperature in each well. The wells have ramps for easy visualisation of the embryos. To secure patient identification, all square dishes in Vitrolife Labware have a dedicated area for labels or barcodes for convenient and safe ID-management. The label area is separate from the handling area.
  • Maintain pH outside the incubator with G-MOPS™
    As this procedure is time consuming and performed outside of the incubator, it is of utmost importance to keep pH stable. G-MOPS™ is a mops-buffered handling medium especially designed to keep pH stable outside the incubator.

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