Slow freezing

Slow freezing
      • The new generation of slow freezing 
        For many years commercially available products for slow freezing were based on variants of the protocol developed by Lasalle and Testart in the 1980s1.With the introduction of FreezeKit Cleave™ and ThawKit Cleave™ we can now offer a completely new procedure for slow freezing based on today’s knowledge of cell physiology and cryopreservation.
      • Metabolism continues during cryopreservation
        As survival is a determining factor in the success after slow freezing2,3 increased survival significantly affects overall efficiency of cryopreservation and ART. FreezeKit Cleave and ThawKit Cleave are based on the G-Series media, with carefully selected amino acids. As metabolism continues during the freezing and thawing procedures, the presence of the amino acids contributes to a less stressful environment and improved embryo viability.
      • Products for different developmental stages
        Embryos can be frozen at all developmental stages. Vitrolife provides kits for freezing and thawing of pronuclear stage oocytes and cleavage stage embryos as well as for blastocysts.
      • REF
        1. High pregnancy rate after early human embryo freezing. Testart J, Lassalle B, Belaisch-Allart J, Hazout A, Forman R, Rainhorn JD, Frydman R.
        2. Survival and developmental potential of stored human early cleavage stage embryos.Edgar DH, Archer J, Gook DA, Jericho H, Wilton L, Bourne H. Eur J Obstet Gynecol Reprod Biol. 2004 Jul 1;115 Suppl 1:S8-11. Review.
        3.  Increasing dehydration of human cleavage-stage embryos prior to slow cooling significantly increases cryosurvival.Edgar DH, Karani J, Gook DA.Reprod Biomed Online. 2009 Oct;19(4):521-5.
      • NOTE. FreezeKit Cleave and ThawKit Cleave are not available for sale in USA