Progressive motility correlates to pregnancy rates
The goal for sperm separation is to select the sperm cells with the highest capacity of fertilising an oocyte. High quality sperm show progressive motility, which in clinical studies has been shown to correlate to pregnancy rates.1,2,3
Clinical evaluation showed increased progressive motility
To investigate the quality of spermatozoa separated with SpermGrad, a clinical evaluation has been performed.The aim was to compare motility of the prepared samples. Semen from 20 patients was included. The samples used for the comparison included severe male factor samples (<5 million/mL initial concentration with motility as low as 10%), as well as normal semen samples.
The data showed that sperm separated using SpermGrad gave a higher proportion of progressively motile sperm in the sample, compared to a leading brand4. This means that SpermGrad facilitates a better separation where a larger proportion of dead and degenerating spermatozoa have been removed.
Designed for gradient separation
The density gradient separation technique is probably the most common method for sperm preparation worldwide. Normal spermatozoa, abnormal spermatozoa, other cells present in the semen sample and bacteria all have different densities. SpermGrad contains silica coated particles that allow for separation of spermatozoa and other cells according to their density.
The density of the gradient determines the result of the separation. One of the many quality tests performed on SpermGrad is density control on every finished LOT, which guarantees consistent product performance.
Assortment meeting your needs
The SpermGrad assortment contains both stock solutions and ready to use solutions, covering the needs from intermittent to high volume use - a time saver in an efficient laboratory.
1. Jouannet P et al. Internation Journal of Andrology, 11:379-394, 1998.
2. Larsen L et al. Human Reproduction, 15:1562-1567, 2000.
3. Zinman MJ et al. Journal of Andrology, 21:145-153, 2000.
4. Meintjes M. Frisco Institute for Reproductive Medicine. Data on file, 2012.