SpermFreeze Solution™

SpermFreeze Solution™

Maintains DNA integrity
Free from egg yolk
Contains only chemically defined components
Ready to use

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SpermFreeze Solution - safe and effective

A study presented at ASRM 20111 compared an egg yolk-free cryopreservation medium from Vitrolife with an egg yolk-containing medium. The focus was to compare the efficacy of the media regarding the following parameters; sperm motility recovery, preservation of sperm-hyaluronic acid binding and preservation of sperm biomarkers that promote paternal contribution of spermatozoa.

The study showed that SpermFreeze Solution and the egg yolk-containing media were equally effective on all tested parameters. SpermFreeze Solution has however the advantage of being egg yolk-free and the risk of transferring diseases from animal derivatives is hence reduced and LOT-to-LOT consistency maintained.

Sperm freezing increases possibility of becoming a parent

In ART, sperm freezing is common among patients with a very low sperm count, those having difficulties producing sperm samples or for those diagnosed with cancer. For these patients, the possibility of becoming a parent depends upon the survival and function of spermatozoa after freezing and thawing procedures.

Developed to maximise sperm survival

SpermFreeze Solution is developed to maximise sperm survival and maintain DNA integrity and function after cryopreservation. SpermFreeze Solution is egg yolk-free and contains only chemically defined components, including glycerol as a cryoprotective agent. The product also contains cholesterol, which has been found to affect the development of acrosomal responsiveness and fertilising ability in vitro2.

Sperm function maintained after cryopreservation

To ensure sperm function after cryopreservation, clinical data was obtained from 48 patients, including ICSI cases and patients receiving conventional IVF treatment3. The study was performed on sibling oocytes and fresh semen was used as a control. The clinical data showed that sperm function was maintained after cryopreservation. There was no statistical difference between the performance of cryopreserved semen compared to fresh semen, see Fig 1.

Table showing data on SpermFreeze Solution
SpermFreeze Solution maintains DNA integrity

Several studies show a negative correlation between defective sperm chromatin structure and fertility, both in vivo4 and in vitro5 . To ascertain that SpermFreeze Solution maintains DNA integrity, semen samples from 22 patients were tested in a sperm chromatin structure assay after cryopreservation using SpermFreeze Solution. The study confirmed that cryopreservation using Vitrolife’s SpermFreeze Solution maintained DNA integrity6.

NOTE: This product is not cleared for sale in US.


1. M. Tekcan et al. P-337. ASRM 2011.
2. Cross. Biology of Reproduction July 1, 1998 vol. 59 no. 1 7-11
3. M H Nasr-Esfahani. Isfahan Fertility and Infertility Center, Iran, 2010.
4. DP Evenson et al. Hum Rep 14, 1999.
5. DP Evenson and Jost LK. Methods Cell Sci 22, 2000.
6. M Bungum. Centre of Reproductive Medicine, Malmö University Hospital, Sweden, 2010.

Product description
Type Description
PurposeFor cryopreservation of human sperm
DescriptionBicarbonate and MOPS buffered medium containing human serum albumin.
ApplicationFor use after equilibration at +20°C ± 5°C and ambient atmosphere.
StorageStore dark at +2 to +8ºC.