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2009-06-25
Oil is important for the outcome of IVF |
| Most clinics today use oil to cover embryo culture media in culture dishes in order to maintain stable temperature, osmolality and pH.
The quality of such oil plays a very important role for the success of IVF. The types of oil commonly used are mineral oil, paraffin oil or a mixture of both these oil types. |
Paraffin oil is more stable than mineral oil |
Paraffin oil and mineral oil are chemically slightly different. The polycarbon lipid tail of mineral oil contains more unsaturated bonds than the more saturated paraffin oil. This makes mineral oil more unstable and prone to attack by free oxygen radicals and photo-oxidation than stable paraffin oil.
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Paraffin oil gives better development |
Bovine embryo development was studied1 in cultures overlaid with either washed mineral or sterile paraffin oil. Sterile filtered paraffin oil overlay resulted in significantly higher (p < 0.05) development rate to morula (44.8% versus 30.6%) and blastocyst (32.8% versus 21.7%) than washed mineral oil.
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Tae, J.C. et al. J. Assist Reprod & Gen. 2006. |
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Superior embryo viability with OVOIL™ from Vitrolife |
Embryo development and viability was evaluated using oils from 6 different manufacturers, see below.
Embryos cultured under OVOIL™ showed significantly better development (p<0.01) on all embryo viability parameters studied; number of cells in the trophectoderm and in the inner cell mass, and total number of cells in the blastocysts.
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REF: D. Linck, SIRT, Australia.
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