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2009-07-01
Improved implantation rate (IR) after lowered pH - Multicentre evaluation of the G5 Series™ before and after pH modification |
| The G5 Series™ media for handling and culture of human embryos was introduced at ESHRE in 2007. In order to verify that the products gave the positive anticipated outcome, a clinical evaluation was initiated. |
According to several studies, intracellular pH in embryos is around 7.2, Vitrolife decided therefore to modify pH of the G5 Series. During the summer of 2008, the upper pH limit for the G5 Series was lowered from 7.40 to a new and more physiological pH range media of 7.20-7.34 at 6% CO2. A second multicenter evaluation started after the pH-change.
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The first evaluation started in August 2007 and ended in December 2007. 15 clinics from 9 countries participated. All clinics were asked to follow the G5 Series manual. 1356 embryos from 585 patients were included. Most embryo transfers were performed at the cleavage stage (529/585) while only a few transfers were performed at the blastocyst stage 56/585. Mean number of cleavage stage embryos for transfer was 1.8 and for blastocysts the mean number was 2.2.
The second multicentre evaluation was performed during the winter of 2008-2009. This time, all clinics worked according to their own laboratory protocols and data was submitted to Vitrolife upon request. 7 clinics from 5 countries provided their data. 1567 embryos from 988 patients were included.
Most embryo transfers were performed at the cleavage stage also in this study (670/988) but considerably more blastocysts transfers were included compared to the previous evaluation, 318/988.
Mean number of cleavage stage embryos for transfer was 1.4 and for blastocysts 1.9. The higher number of blastocysts transferred compared to cleavage stage embryos, is due to the policy of the participating clinics.
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The implantation rate for Day 2/3 transfers increased from 26% to 34% and for blastocyst transfers the implantation rate increased from 35% to 40%. The increase in implantation rate for cleavage stage embryos was significant, p<0.001.
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A lower and more physiological pH-range has significantly improved implantation rates for day 2/3 embryos (p<0.001). For blastocysts the higher implantation level did not reach significance due to the low number of patients who participated in the evaluation 2007.
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REF. Vitrolife Data on file. 2009 |
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