A retrospective study of single step time-lapse medium (Vitrolife, G-TL) and sequential series media (Sage, Quinn’s advantage) in 4 well (1 ml) dishes (N=49; N=65) and Primo Vision time-lapse dishes (N=16; N=25) with embryos donated to research.
There was no significant difference (p=0.38) at an average of 106 hpi (hours post insemination) in G-TL (N=66) and 106.7 hpi in QA (N=56). Neither did the rates of glucose consumption show a significant difference between the two media systems (p=0.56)
The blastocyst development rate in the G-TL group was significantly higher in both large and small volume culture, see graph.
Blastocyst development day 5
When using G-TL for purpose, using micro droplet culture and Primo Vision time-lapse system, and comparing to standard sequential media, blastocyst development was significantly improved, despite that time to blastocoel formation did not differ significantly.
The authors conclude that the optimum culture media to support embryo viability is G-TL in Primo Vision culture dishes.
Ref: L.A. Shaw et al. ACE 2015 poster