- HYASE-10X™ for removal of cumulus cells
To be able to reach the oocyte and fulfill the mission of fertilisation, the spermatozoa release the enzyme hyaluronidase to make their way through the cumulus mass. In vitro, if ICSI is used as a fertilisation method, the cumulus cells must be removed by the embryologist. The same enzyme released by the spermatozoa is used. When oocytes are placed in diluted HYASE- 10X the cumulus mass is dispersed in a few seconds and the cells can easily be removed with a denudation pipette.
- Recombinant albumin for safety
To immobilize and catch a quick swimming sperm for ICSI is a challenge. ICSI is a highly viscous solution for the immobilization of spermatozoa. It contains recombinant human albumin for safety and the most widely used molecule used for this purpose, polyvinylpyrrolidone or PVP. PVP is an inert molecule that does not take part in any metabolic pathway.
- Vitrolife has long experience with ICSI
ICSI from Vitrolife was the first product on the market designed exclusively for the ICSI procedure and introduced already in 1993-1994. Before the introduction of this product the clinics working with ICSI had to either use impure PVP or employ dialysis to purify PVP themselves, a very cumbersome procedure. PVP has been used for ICSI since the start of this fertilisation method which means that the vast majority of children born after ICSI has been conceived with the assistance of PVP.