SpermGrad™

Efficient separation for increased progressive motility.

Purpose For gradient sperm separation.

Storage Store dark at +2 to +8°C

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10099 SpermGrad™
  • Description: Bicarbonate and HEPES buffered medium containing silane-coated, colloid silica particles.
  • Application: For use after dilution with either G-IVF™ supplemented with G-MM™ or HSA-solution™ , or with SpermRinse™ .
10102 SpermGrad™
  • Description: Bicarbonate and HEPES buffered medium containing silane-coated, colloid silica particles.
  • Application: For use after dilution with either G-IVF™ supplemented with G-MM™ or HSA-solution™ , or with SpermRinse™ .
10138 SpermGrad™
  • Description: Bicarbonate and HEPES buffered medium containing silane-coated, colloid silica particles. Upper (45%) and lower (90%) layer.
  • Application: Ready to use solutions for gradient sperm separation.
10139 SpermGrad™
  • Description: Bicarbonate and HEPES buffered medium containing silane-coated, colloid silica particles. Upper (45%) and lower (90%) layer.
  • Application: Ready to use solutions for gradient sperm separation.
10238 SpermGrad™ (Lower layer, 90%)
  • Description: Bicarbonate and HEPES buffered medium containing silane-coated, colloid silica particles.
  • Application: Ready to use solutions for gradient sperm separation.
10239 SpermGrad™ (Lower layer, 90%)
  • Description: Bicarbonate and HEPES buffered medium containing silane-coated, colloid silica particles.
  • Application: Ready to use solutions for gradient sperm separation.
10338 SpermGrad™ (Upper layer, 45%)
  • Description: Bicarbonate and HEPES buffered medium containing silane-coated, colloid silica particles.
  • Application: Ready to use solutions for gradient sperm separation.
10339 SpermGrad™ (Upper layer, 45%)
  • Description: Bicarbonate and HEPES buffered medium containing silane-coated, colloid silica particles.
  • Application: Ready to use solutions for gradient sperm separation.

Assortment that meets your need

The SpermGrad assortment contains both stock solutions and ready to use solutions, covering the needs from intermittent to high volume use - a time saver in an efficient laboratory. The stock solution is to be used after dilution with either G-IVF™ supplemented with G-MM™ or HSA-solution™, or with SpermRinse™.

Progressive motility correlates
to pregnancy rates

The goal for sperm separation is to select the sperm cells with the highest capacity of fertilising an oocyte. High quality sperm show progressive motility, which in clinical studies has been shown to correlate to pregnancy rates.1,2,3

Normal spermatozoa, abnormal spermatozoa, other cells present in the semen sample and bacteria all have different densities. SpermGrad contains silica coated particles that allow for separation of spermatozoa and other cells according to their density.

REF: 1. Jouannet P et al. Internation Journal of Andrology, 11:379-394, 1998. 2. Larsen L et al. Human Reproduction, 15:1562-1567, 2000. 3. Zinman MJ et al. Journal of Andrology, 21:145-153, 2000.

Clinical evaluation showed increased progressive motility

To investigate the quality of spermatozoa separated with SpermGrad, a clinical evaluation has been performed. The aim was to compare motility of the prepared samples and semen from 20 patients was included. The samples used for the comparison included severe male factor samples (<5 million/mL initial concentration with motility as low as 10%), as well as normal semen samples.

The data showed that sperm separated using SpermGrad gave a higher proportion of progressively motile sperm in the sample, compared to a leading brand1. This means that SpermGrad facilitates a better separation where a larger proportion of dead and degenerating spermatozoa have been removed.

REF: 1. Meintjes M. Frisco Institute for Reproductive Medicine. Data on file, 2012.

Expand your knowledge about sperm

Broaden your knowledge about sperm and their contribution to the oocyte by watching the two lectures by a leading authority in the field, Prof. Christopher Barratt from University of Dundee. He describes some of the latest research about sperm and oocyte interaction.

 
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How to use this product

Package inserts
SpermGrad™

Package inserts

Package insert SpermGrad

Product manuals
SpermGrad™

Product manuals

Manual G-Series. International

Product manuals
SpermGrad™

Product manuals

Manual G-Series. For US

Additional documents

Short protocols
SpermGrad™

Short protocols

Short protocols G-Series Sperm preparation - density gradient

This short protocol describes how to perform sperm preparation (density gradient method) using Vitrolife products.

Scientific summaries
SpermGrad™

Scientific summaries

Sperm preparation with SpermGrad show positive effect on average telomere length, sperm motility and DNA fragmentation

Yang Q, et al, found significant correlations between sperm telomere length and the quality of early embryonic development. Density gradient centrifugation using SpermGrad (Vitrolife) enrich for sperm with high mobility and low DNA fragmentation and is the preferred technique for selection of sperm with longer telomeres.

Safety data sheets (SDS)
SpermGrad™

Safety data sheets (SDS)

SDS SpermGrad

Safety data sheet for SpermGrad

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