OVOIL™ - culture oil

100% paraffin oil produced in a highly controlled process from extensively tested and selected LOTs of raw material.

Description Light paraffin oil, sterile filtered.

Purpose Oil for covering of medium during in vitro fertilisation and micromanipulation procedures.

Application For use after pre-equilibration at +37ºC and 5 % CO2, 6 % CO2 or ambient atmosphere, depending on the intended use.

Storage Store dark at +2 to +8°C

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10029 OVOIL™ - culture oil

The importance of oil for IVF outcome

Most clinics today use oil to cover embryo culture dishes in order to maintain stable temperature, osmolality and pH. The quality of such oil plays a very important role for IVF success. The oil types commonly used are mineral oil, paraffin oil or a mixture of both these oil types. 

Paraffin oil and mineral oil are chemically slightly different. The polycarbon lipid tail of mineral oil contains more unsaturated bonds than the more saturated paraffin oil. This makes mineral oil more unstable and prone to attack by free oxygen radicals and photooxidation than paraffin oil.

Read this blog post to learn more about the characteristics of the perfect oil for IVF.

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Comparison between mineral oil and paraffin oil

In a study by Tae et al. bovine embryo development was studied in cultures overlaid with either washed mineral or sterile paraffin oil.

Sterile filtered paraffin oil overlay resulted in significantly higher (p<0.05) development rate to morula (44.8% versus 30.6%) and blastocyst (32.8% versus 21.7%) than washed mineral oil.

REF: Tae, J.C. et al. J. Assist Reprod & Gen. 2006

The importance of peroxide-free oil for embryo culture

Download a scientific summary from Japanese researcher Otsuki to learn more about why high peroxide levels in oil can harm the culture system and the results from peroxide values in oil from different manufacturers.

Download summary

Oil is not just oil

Oils from six different manufacturers were tested and embryo development and viability was evaluated.

OVOIL showed significantly better development (p<0.01) for all embryo viability parameters studied, number of cells in the trophectoderm and in the inner cell mass, and total number of cells in the blastocysts.

REF: D. Linck, SIRT, Australia. 2008.

OVOIL - extensively quality tested

  • Sperm sensitivity testing
  • 1-cell mouse embryo assays
    • Sensitive CF1 mouse strain on raw material
    • 1 cell, F1 hybrid mouse on finished product
    • Mouse embryo assays with multiple endpoints
    • Blastocyst differentiation staining and cell counts

Read more on how to ensure embryo viablity in your culture system:

Read blog post

Optimise all the way with G-Series

Ensure optimal conditions before, during and after culture by using the whole G-Series™. All G-Series media share the same basic composition to secure viability and implantation potential. Osmolality, pH and supporting compounds are all kept constant. This foundation prevents intracellular stresses as the embryos progress through the IVF process.

How to use this product

Package inserts
OVOIL™ - culture oil

Package inserts

Package insert OVOIL

Product manuals
OVOIL™ - culture oil

Product manuals

Manual G-Series. International

Product manuals
OVOIL™ - culture oil

Product manuals

Manual G-Series. For US

Additional documents

Short protocols
OVOIL™ - culture oil

Short protocols

Short protocol - G-Series - Embryo culture

This short protocol describes how to perform embryo culture using the G-Series products

Short protocols
OVOIL™ - culture oil

Short protocols

Short protocol - Embryo culture with G-1, G-2 and Primo Vision - Method 1

This short protocol describes how perform time-lapse culture with PrimoVision using G-1 and G-2. Change of media on day 3 is done by preparing a new dish and transfer the embryos.

Short protocols
OVOIL™ - culture oil

Short protocols

Short protocol - Embryo culture with G1, G2 and Primo Vision - Method 2

This short protocol describes how to perform time-lapse culture using G-1, G-2 and Primo Vision. Change of media on day 3 is done removing G-1 in the dish and adding G-2.

Short protocols
OVOIL™ - culture oil

Short protocols

Short protocol - Embryo culture with G-TL and Primo Vision

This short protocol describes how to perform time-lapse culture using G-TL and PrimoVision

Short protocols
OVOIL™ - culture oil

Short protocols

Short protocol - G-Series - Blastocyst trophectoderm biopsy

This short protocol describes how to perform blastocyst trophectoderm biopsy using G-2™ PLUS or G-TL™, OVOIL™ and G-MOPS™ PLUS

Scientific summaries
OVOIL™ - culture oil

Scientific summaries

Comparison of cover oils - OVOIL gives both more good and top quality embryos

In a study by Sifer et el, microdroplet embryo cultures covered with OVOIL from Vitrolife gave a significantly increased mean number of good and top quality embryos at day 3, compared to other commercial available oils. This difference may be explained by the origin of raw materials and the difference in quality tests performed by each company.

Safety data sheets (SDS)
OVOIL™ - culture oil

Safety data sheets (SDS)

SDS OVOIL

Safety data sheet for OVOIL

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