OVOIL™ - culture oil
100% paraffin oil produced in a highly controlled process from extensively tested and selected LOTs of raw material.
Description Light paraffin oil, sterile filtered.
Purpose Oil for covering of medium during in vitro fertilisation and micromanipulation procedures.
Application For use after pre-equilibration at +37ºC and 5 % CO2, 6 % CO2 or ambient atmosphere, depending on the intended use.
Storage Store dark at +2 to +8°C
The importance of oil for IVF outcome
Most clinics today use oil to cover embryo culture dishes in order to maintain stable temperature, osmolality and pH. The quality of such oil plays a very important role for IVF success. The oil types commonly used are mineral oil, paraffin oil or a mixture of both these oil types.
Paraffin oil and mineral oil are chemically slightly different. The polycarbon lipid tail of mineral oil contains more unsaturated bonds than the more saturated paraffin oil. This makes mineral oil more unstable and prone to attack by free oxygen radicals and photooxidation than paraffin oil.
Read this blog post to learn more about the characteristics of the perfect oil for IVF.Read blog post
Comparison between mineral oil and paraffin oil
In a study by Tae et al. bovine embryo development was studied in cultures overlaid with either washed mineral or sterile paraffin oil.
Sterile filtered paraffin oil overlay resulted in significantly higher (p<0.05) development rate to morula (44.8% versus 30.6%) and blastocyst (32.8% versus 21.7%) than washed mineral oil.
The importance of peroxide-free oil for embryo culture
Download a scientific summary from Japanese researcher Otsuki to learn more about why high peroxide levels in oil can harm the culture system and the results from peroxide values in oil from different manufacturers.Download summary
Oil is not just oil
Oils from six different manufacturers were tested and embryo development and viability was evaluated.
OVOIL showed significantly better development (p<0.01) for all embryo viability parameters studied, number of cells in the trophectoderm and in the inner cell mass, and total number of cells in the blastocysts.
OVOIL - extensively quality tested
- Sperm sensitivity testing
- 1-cell mouse embryo assays
- Sensitive CF1 mouse strain on raw material
- 1 cell, F1 hybrid mouse on finished product
- Mouse embryo assays with multiple endpoints
- Blastocyst differentiation staining and cell counts
Read more on how to ensure embryo viablity in your culture system:Read blog post
Optimise all the way with G-Series
Ensure optimal conditions before, during and after culture by using the whole G-Series™. All G-Series media share the same basic composition to secure viability and implantation potential. Osmolality, pH and supporting compounds are all kept constant. This foundation prevents intracellular stresses as the embryos progress through the IVF process.
How to use this product
Manual G-Series. For US
This manual describes how to use all media in the G-Series: G-RINSE, G-MOPS, G-GAMETE, G-IVF, G-1, G-2, G-TL, G-PGD, HSA-solution, G-MM, ICSI, HYASEx-10x, OVOIL, SpermGrad and EmbryoGlue
Short protocol - Embryo culture
This short protocol describes how to culture embryos from after fertilisation until the blastocyst stage using the G-Series™ sequential media G-1™ PLUS, G-2™ PLUS and OVOIL™.
Short protocol - Embryo Culture using sequential media and Primo Vision™ time-lapse embryo monitoring system – Method 1
This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for handling of oocytes and embryo culture in Primo Vision™ time-lapse monitoring system and change medium on day 3 by preparing a new dish.
Short protocol - Embryo Culture using sequential media and Primo Vision™ time-lapse embryo monitoring system – Method 2
This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS™ PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for oocyte handling and embryo culture in Primo Vision time-lapse monitoring system and change medium on day 3 using the same dish.
Short protocol - Embryo Culture using G-TL™ with Primo Vision™ Time-lapse monitoring system
This short protocol describes handling of embryos for culture in Primo Vision time-lapse system using G-Series ™media G-MOPS™, G-MOPS™ PLUS, OVOIL™ and G-TL™.
Short protocol - Blastocyst trophectoderm biopsy
This short protocol describes how to perform blastocyst trophectoderm biopsy using OVOIL™ and G-Series™ media, G-2™ PLUS or G-TL™ and G-MOPS™ PLUS
Short protocols - ICSI
This short protocol describes how to perform ICSI using ICSI™, HYASE™-10x, OVOIL™ and G-Series medium G-MOPS™ PLUS
Comparison of cover oils - OVOIL gives both more good and top quality embryos
In a study by Sifer et el, microdroplet embryo cultures covered with OVOIL from Vitrolife gave a significantly increased mean number of good and top quality embryos at day 3, compared to other commercial available oils. This difference may be explained by the origin of raw materials and the difference in quality tests performed by each company.