OVOIL HEAVY™
A high viscosity oil offering potential for improved handling and embryo viability.
Description OVOIL HEAVY is a high viscosity oil, sterile filtered.
Purpose Oil for covering of medium during in vitro fertilisation and micromanipulation procedures.
Application For use after equilibration at +37°C in CO2-incubator or ambient atmosphere, depending on the intended use.
Storage Store dark at +2 to +8°C. During transportation temperature may exceed +8°C but not +25°C, for a maximum of seven days.
OVOIL HEAVY™ is not just any other oil.
OVOIL HEAVY is the world’s first synthetically derived paraffin oil – developed, manufactured, tested and certified specifically for clinical IVF. With consistently high lot-to-lot quality and increased viscosity, OVOIL HEAVY™ provides exceptional safety for your embryo culture while improving handling qualities in the lab.
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The importance of oil for IVF outcome
Oil for IVF should do nothing but protect the culture. It should not have any negative influence on the gametes, embryos or media. Most clinics today use oil to cover embryo culture dishes in order to maintain stable temperature, osmolality and pH. The quality of such oil plays a very important role in IVF success. The oil types commonly used are mineral oil, paraffin oil or a mixture of both.
Paraffin and mineral oil are chemically slightly different. The polycarbon backbone of mineral oil contains double bonds. This makes it more unstable and prone to attack by free oxygen radicals and photo-oxidation than paraffin oil.
Improved handling thanks to higher viscosity
It is important to have an oil that is easy to handle in the laboratory. One of the main benefits of OVOIL HEAVY™ is its higher viscosity, which in turn provides significantly improved handling qualities in the lab.
Extensive quality testing
OVOIL HEAVY™ is an extensively quality tested product:
- Human sperm oil assay
- 1-cell mouse embryo assays
- Sensitive CF1 mouse strain on raw material
- 1 cell, F1 hybrid mouse on finished product
- Mouse embryo assays with multiple endpoints for
grading development - Blastocyst differentiation staining and cell counts
- Micro droplet oil test
We have been given the opportunity to test Ovoil Heavy at the American Hospital of Paris. Happy users of the regular OVOIL for years we could not imagine to find a higher quality product. In fact Ovoil Heavy happened to be a more easy to use product. It's high viscosity helps avoiding splashing and spilling during dish preparation and handling still with the same premium quality. Ovoil Heavy met all our expectations and we decided to switch from the regular Ovoil to the heavy version.
- Dr. Frida Entezami, Lab manager of American Hospital of Paris
When comparing Ovoil Heavy to former oils I felt it offers me as an embryologist many handling benefits. I like the viscosity, how easy it is to pipette and there is much less spilling when using it. The higher viscosity allows easier control when injecting sperm during ICSI. I trust this oil knowing that it is heavily quality tested with specific MEA for oil. Having visited the Vitrolife lab and observing the process of quality control that were used to manufacture their products I can 100% confidently recommend their heavy oil.
- Van Pham, Chief Embryologist at Aspire Fertility in Houston, The Prelude Network
How to use this product
Additional documents
Short protocols
Short protocol - Embryo culture
This short protocol describes how to culture embryos from after fertilisation until the blastocyst stage using the G-Series™ sequential media G-1™ PLUS, G-2™ PLUS and OVOIL™.
Short protocols
Short protocol - Embryo Culture using sequential media and Primo Vision™ time-lapse embryo monitoring system – Method 1
This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for handling of oocytes and embryo culture in Primo Vision™ time-lapse monitoring system and change medium on day 3 by preparing a new dish.
Short protocols
Short protocol - Embryo Culture using sequential media and Primo Vision™ time-lapse embryo monitoring system – Method 2
This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS™ PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for oocyte handling and embryo culture in Primo Vision time-lapse monitoring system and change medium on day 3 using the same dish.
Short protocols
Short protocol - Embryo Culture using G-TL™ with Primo Vision™ Time-lapse monitoring system
This short protocol describes handling of embryos for culture in Primo Vision time-lapse system using G-Series ™media G-MOPS™, G-MOPS™ PLUS, OVOIL™ and G-TL™.
Short protocols
Short protocol - Blastocyst trophectoderm biopsy
This short protocol describes how to perform blastocyst trophectoderm biopsy using OVOIL™ and G-Series™ media, G-2™ PLUS or G-TL™ and G-MOPS™ PLUS
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