A high viscosity oil offering potential for improved handling and embryo viability.
Description OVOIL HEAVY is a high viscosity oil, sterile filtered.
Purpose Oil for covering of medium during in vitro fertilisation and micromanipulation procedures.
Application For use after equilibration at +37°C in CO2-incubator or ambient atmosphere, depending on the intended use.
Storage Store dark at +2 to +8°C. During transportation temperature may exceed +8°C but not +25°C, for a maximum of seven days.
OVOIL HEAVY™ is not just any other oil.
OVOIL HEAVY is the world’s first synthetically derived paraffin oil – developed, manufactured, tested and certified specifically for clinical IVF. With consistently high lot-to-lot quality and increased viscosity, OVOIL HEAVY™ provides exceptional safety for your embryo culture while improving handling qualities in the lab.Read blog post
The importance of oil for IVF outcome
Oil for IVF should do nothing but protect the culture. It should not have any negative influence on the gametes, embryos or media. Most clinics today use oil to cover embryo culture dishes in order to maintain stable temperature, osmolality and pH. The quality of such oil plays a very important role in IVF success. The oil types commonly used are mineral oil, paraffin oil or a mixture of both.
Paraffin and mineral oil are chemically slightly different. The polycarbon backbone of mineral oil contains double bonds. This makes it more unstable and prone to attack by free oxygen radicals and photo-oxidation than paraffin oil.
Improved handling thanks to higher viscosity
It is important to have an oil that is easy to handle in the laboratory. One of the main benefits of OVOIL HEAVY™ is its higher viscosity, which in turn provides significantly improved handling qualities in the lab.
Extensive quality testing
OVOIL HEAVY™ is an extensively quality tested product:
- Human sperm oil assay
- 1-cell mouse embryo assays
- Sensitive CF1 mouse strain on raw material
- 1 cell, F1 hybrid mouse on finished product
- Mouse embryo assays with multiple endpoints for
- Blastocyst differentiation staining and cell counts
- Micro droplet oil test
How to use this product
Short protocol - Embryo culture
This short protocol describes how to culture embryos from after fertilisation until the blastocyst stage using the G-Series™ sequential media G-1™ PLUS, G-2™ PLUS and OVOIL™.
Short protocol - Embryo Culture using sequential media and Primo Vision™ time-lapse embryo monitoring system – Method 1
This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for handling of oocytes and embryo culture in Primo Vision™ time-lapse monitoring system and change medium on day 3 by preparing a new dish.
Short protocol - Embryo Culture using sequential media and Primo Vision™ time-lapse embryo monitoring system – Method 2
This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS™ PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for oocyte handling and embryo culture in Primo Vision time-lapse monitoring system and change medium on day 3 using the same dish.
Short protocol - Embryo Culture using G-TL™ with Primo Vision™ Time-lapse monitoring system
This short protocol describes handling of embryos for culture in Primo Vision time-lapse system using G-Series ™media G-MOPS™, G-MOPS™ PLUS, OVOIL™ and G-TL™.
Short protocol - Blastocyst trophectoderm biopsy
This short protocol describes how to perform blastocyst trophectoderm biopsy using OVOIL™ and G-Series™ media, G-2™ PLUS or G-TL™ and G-MOPS™ PLUS