G-2™

Document ref and version: REF 26082, version 11

Document ref and version: Document and Ref: Date Revised : 04/16/2025.Revision No : 7. Safety data sheet for G-2 PLUS

Document ref and version: REF 19183. Version 07. Date of issue 220923. This short protocol describes how to culture embryos from after fertilisation until the blastocyst stage using the G-Series™ sequential media G-1™ PLUS, G-2™ PLUS and OVOIL™.

Document ref and version: REP-3357. Version 6.0. 2025/04/10

Document ref and version: A study by Zhu et al demonstrate that extended culture before cryopreservation allows for selection of viable embryos with a high chance of implantation after warming. The high blastocyst formation rate (73.8%) using Vitrolife culture media, together with an efficient cryopreservation program resulting in survival rates of >96% should eliminate any earlier concerns about blastocyst culture and cryopreservation.

Document ref and version: A study by Kleijkers show that besides cell numbers and implantations rates, gene expression and certain pathways in preimplantation embryos are also affected by culture medium.

Document ref and version: REF 19126. Version 02. Date of issue 220923. This short protocol describes how to perform blastocyst trophectoderm biopsy using OVOIL™ and G-Series™ media, G-2™ PLUS or G-TL™ and G-MOPS™ PLUS

Document ref and version: In a study by Matsuoka et al, embryo culture was done either Vitrolife’s G-Series™ culture media or a mix of sequential culture media (control). The results show that good-quality blastocyst development was more frequent after culture in G-Series media containing hyaluronan.