Short protocols

Short protocols - Embryo transfer

This short protocol describes how to perform embryo transfer using EmbryoGlue® 5x1.5 ml

Short protocol - Rapid-i™ Vitrification Omni. For all stages, from oocytes to blastocysts

This short protocol describes how to vitrify and warm all cell stages from oocytes to blastocysts using Rapid-i ™vitrification system and RapidVit™/Warm™ Omni. REF 19105 Version 02. Date of issue 20220923

Short protocol - Embryo culture Gx Series

This short protocol describes how to culture embryos from after fertilisation until the blastocyst stage using the G-Series™ sequential media G-1™ PLUS, G-2™ PLUS and OVOIL™.

Short protocol - Embryo Culture using sequential media and Primo Vision™ time-lapse embryo monitoring system – Method 1

This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for handling of oocytes and embryo culture in Primo Vision™ time-lapse monitoring system and change medium on day 3 by preparing a new dish.

Short protocol - Embryo Culture using sequential media and Primo Vision™ time-lapse embryo monitoring system – Method 2

This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS™ PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for oocyte handling and embryo culture in Primo Vision time-lapse monitoring system and change medium on day 3 using the same dish.

Short protocol - SPERM FREEZING Sperm freezing when using cryovials

This short protocol describes freezing of semen when using vials followed by gradient separation using and G-Series™ medium G-IVF™ PLUS and SpermGrad. ™

Short protocol - SPERM FREEZING Sperm freezing when using straws

This short protocol describes freezing of semen when using straws followed by gradient separation using G-Series™ medium G-IVF™ PLUS and SpermGrad™.

Short protocol - Dish loading Primo Vision™ time-lapse system

This short protocol describes how to load Primo Vision™ dishes using 9-16 well dish.

Short protocol - Hardware set up Primo Vision™ time-lapse system

This short protocol describes how to set up Primo Vision™ time-lapse monitoring system in a box incubator and connect to the computer.

Short protocol - Start new project Primo Vision™ time-lapse system

This short protocol describes how to start a new project (patient) in Primo Vision™ time-lapse monitoring system.

Short protocol - Rapid-i™ Vitrification System of blastocysts

This short protocol describes how to vitrify and warm blastocysts using Rapid-i™ vitrification system and RapidVit™/RapidWarm™ Blast. REF 19128. Version 03. Date of issue 220923.

Short protocol - Rapid-i™ Vitrification of Cleavage stage embryos

This short protocol describes how to vitrify and warm cleavage stage embryos using Rapid-i™ vitrification system and RapidVit™/RapidWarm ™ Cleave. REF 19127. Version 02. Date of issue 20220923.

Short protocol - Rapid- i™ Vitrification of oocytes

This short protocol describes how to vitrify and warm oocytes using Rapid-i™ vitrification system and RapidVit™/RapidWarm™ Oocyte. REF 19129. Version 03. Date of issue 220923.

Short protocols - Cryo preservation Cleavage stage embryos

This short protocol describes how to slow-freeze zygotes and cleavage stage embryos using FreezeKit™ & ThawKit™ Cleave.

ICSI Procedure

Gx Series

G-TL Embryo Culture

REF 19125 version 1. Date of issure 2019-08-12

Gx-TL Embryo Culture

1.181023.2

Cryopreservation

Blasotcyst stage

Short protocol - Embryo transfer

This short protocol describes how to perform embryo transfer using EmbryoGlue®.

Blastocyst trophectoderm biopsy

Gx Series

Embryo culture GX-TL

Gx Series

Oocyte retrieval and fertilisation

Gx Series

Sperm preparation Gx Series

Density gradient centrifugation method

Short protocol - Blastocyst trophectoderm biopsy

This short protocol describes how to perform blastocyst trophectoderm biopsy using OVOIL™ and G-Series™ media, G-2™ PLUS or G-TL™ and G-MOPS™ PLUS

Short protocol - Embryo Culture using G-TL™ with Primo Vision™ Time-lapse monitoring system

This short protocol describes handling of embryos for culture in Primo Vision time-lapse system using G-Series ™media G-MOPS™, G-MOPS™ PLUS, OVOIL™ and G-TL™.

Short protocols - ICSI

This short protocol describes how to perform ICSI using ICSI™, HYASE™-10x, OVOIL™ and G-Series medium G-MOPS™ PLUS

Short protocols - Oocyte retrieval and fertilisation assessment

This short protocol describes handling of oocytes and embryos in ambient atmosphere using G-Series™ media G-RINSE™, G-MOPS™, G-MOPS™ PLUS and G-IVF™ PLUS

Short protocols - Sperm preparation, Density gradient centrifugation method

This short protocol describes how to perform sperm preparation with the density gradient method and fertilization using products G-Series™ medium G-IVF™ PLUS and SpermGrad™

Short protocols - Sperm preparation, Swim up method

This short protocol describes how to perform sperm preparation with the swim-up method and fertilization of oocytes using G-series™ medium G-IVF™ PLUS

ICSI procedure

Short protocols - Sperm preparation

Density gradient centrifugation method