Culture
How to use these products
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Embryo culture using G-1 and G-2
This movie demonstrates how to perform embryo culture using culture media G-1, G-2, G-MOPS PLUS and OVOIL.
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Embryo culture using G-TL and Primo Vision time-lapse system
This movie demonstrates how to perform embryo culture in a time-lapse setting using the culture media G-TL, specifically designed for time-lapse culture, together with Primo Vision time-lapse system. G-MOPS PLUS and OVOIL are also part of the procedure.
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Preparation of CulturePro dishes
Learn how to prepare and load the CulturePro dishes for the CulturePro incubator in this video.
Read up on studies
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G-Series culture media improve blastocyst development
Data from two studies presented at Annual Meeting of the Japanese Society of Clinical Embryologist show that good quality embryo rate and blastocyst development can be improved when using G-Series culture media (G-IVF, G-1 and G-2) from Vitrolife compared to a HTF-based non-hyaluronan containing media.
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Embryo culture in G-Series results in significantly higher cell numbers and implantation rates compared to culture in HTF
A study by Kleijkers show that besides cell numbers and implantation rates, gene expression and certain pathways in preimplantation embryos are also affected by culture medium.
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Comparison of cover oils - OVOIL gives both more good and top quality embryos
In a study by Sifer et el, microdroplet embryo cultures covered with OVOIL from Vitrolife gave a significantly increased mean number of good and top quality embryos at day 3, compared to other commercial available oils. This difference may be explained by the origin of raw materials and the difference in quality tests performed by each company.
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The benefits of using a peroxide-free oil
Japanese researcher Otsuki and colleagues experienced reduction in fertilisation rate and impaired embryo development when they began to use a new bottle of oil at their IVF clinic. They decided to make an investigation of the root cause and found it to be high peroxide levels in the oil. Download a summary of their findings to learn more about why high peroxide levels in oil can harm the culture system and the results from peroxide values in oil from different manufacturers.
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G-TL supports blastocyst development in large volume
In a study presented at the ACE meeting in the UK, Shaw and co-workers compared single step time-lapse medium (Vitrolife, G-TL) with sequential series media (Sage, Quinn’s advantage) in large volume culture as well as micro drop culture. They found significantly higher day 5 blastocyst development rate in the G-TL group in both large and small volume culture.
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G-Series culture media shown to increase blastocyst development compared to control
In a study by Matsuoka et al, embryo culture was done either Vitrolife’s G-Series™ culture media or a mix of sequential culture media (control). The results show that good-quality blastocyst development was more frequent after culture in G-Series media containing hyaluronan.
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Optimised clinical outcomes with blastocyst culture and cryopreservation using Vitrolife G-Series culture media
A study by Zhu et al demonstrate that extended culture before cryopreservation allows for selection of viable embryos with a high chance of implantation after warming. The high blastocyst formation rate (73.8%) using Vitrolife culture media, together with an efficient cryopreservation program resulting in survival rates of >96% should eliminate any earlier concerns about blastocyst culture and cryopreservation.
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Clinical evaluation and user experience of Vitrolife Labware dishes
Five dishes specifically developed and toxicity tested for IVF were evaluated by two clinics regarding clinical performance. The results at both clinics show that Vitrolife Labware are safe and support treatment success when used for clinical IVF. In addition, twenty clinics gave the dishes a higher score of usability than their traditional dishes showing that the new dish design is at least as user friendly as other dish types commonly used for IVF.
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Increase my knowledge
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Amino acids and ART
Read this blog post where Prof. David Gardner appreciates amino acids and the highly diverse roles they play in regulating embryo physiology. Here their functions are considered and how their supplementation to embryo culture media has contributed significantly to the increase in IVF success rates worldwide.
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How to ensure embryo viability in your culture system
Successful outcomes in IVF require optimal environmental conditions for gametes and embryos throughout the entire cycle. This can be accomplished by ensuring optimal pH and temperature in the incubators, and that the air in the operating theater and the laboratory is clean and free of VOC’s (volatile organic compounds). An additional step is to ensure that media and contact materials used, support embryo development by being embryo safe and free from toxicity.
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Vitrolife Labware - for improved culture conditions
Listen to Tony Winslöf presenting Vitrolife Labware, a series of products from Vitrolife created, dedicated and certified for use in IVF. Vitrolife Labware has been developed in close collaboration with IVF clinics with the aim of simplifying and improving daily work in the lab.
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Do you know the characteristics of the perfect oil for IVF?
Perfect oil for IVF should do nothing but protect the culture. It should not have any negative influence on the gametes, embryos or media by adding unwanted or removing necessary components. However not all oils are or act the same. In this blog you will learn about different types of oil and things to consider when choosing oil for your IVF culture.
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How to create optimal culture conditions by paying attention to quality control
The human embryo is extremely sensitive to stress and surrounding factors. In fact, the embryonic stage is the most sensitive stage of the whole life cycle. This is why it is particularly important to use safe and non-toxic products during gamete handling and embryo culture.
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Ways to minimise stress to facilitate viable embryo development
In this lecture from ESHRE 2014, Prof David Gardner discusses different types of stresses to the human developing embryo and presents ways create environments and media that restrict the impact of such in vitro-induced stresses.
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