The new generation of slow freezing – optimised composition, convenient protocol and high survival rate.
Description MOPS buffered media.
Application For use in sequence after equilibration at room temperature and ambient atmosphere.
Storage Store dark at +2 to +8°C.
FreezeKit Cleave* and ThawKit Cleave* have been developed for freezing and thawing of pronuclear oocytes and cleavage stage embryos.
*US only: For freezing and thawing of pronuclear (2PN) and cleavage stage embryos.
FreezeKit Cleave and ThawKit Cleave make the whole slow freezing procedure faster and more convenient. With fewer steps compared to the current protocol they save time, reduce operator variability and simplify the training process.
Most importantly, embryos are exposed to less stress with the fewer steps and shorter handling times.
Two clinics in Sweden made a pre-clinical, randomised comparison of FreezeKit Cleave and ThawKit Cleave with an existing commercial product for freezing of cleavage-stage embryos.
The primary endpoint was blastomere survival rate. A statistically significant difference in blastomere survival was seen between the two methods.
Read guest blogger professor David Edgar's blog post about slow freezing and its role in cryopreservation.
Read blog post
Package insert ThawKit Cleave
Package insert FreezeKit Cleave US
Package insert ThawKit Cleave US
Short protocols Slowfreezing - cleavage stage embryos
This short protocol describes how to perform slowfreezing on cleavage stage embryos using FreezeKit Cleave and ThawKit Cleave.
New formulations of slowfreezing solutions for cleavage stage embryos result in outcomes similar to after vitrification.pdf
A study by Fang et al, found that FreezeKit Cleave and ThawKit Cleave significantly improved survival rates, clinical pregnancy rates and implantation rates. In fact, a survival rate of above 90% using the new formulations of solutions is very similar to that obtained after vitrification.