To be successful with vitrification and exert minimal effect on cell viability, a balance between cryoprotectant concentration, cooling and warming rates as well as volume is necessary.
Our solutions puts you in control. The method, media, device and accessories are all you need to simplify your workflow – from vitrification and storage to warming of oocytes as well as embryos.
We provide media for vitrification and warming of all stages, from oocytes to blastocysts.
Both vitrification and warming are performed at physiological temperature (37°C). This helps to maintain the spindle integrity and viability of oocytes and embryos. Working at physiological temperatures shortens the vitrification time and minimises exposure to cryoprotectants and their potential toxic effects.
Using RapidVit and RapitWarm Omni will save you time in every procedure.
The graphs compare the maximum times needed to vitrify using different brands.
Rapid-i™ is a unique vitrification device. After loading of the embryos onto the Rapid-i, the device is inserted into a pre-cooled RapidStraw™. The air inside the straw is super-cooled by the surrounding liquid nitrogen and the vitrification occurs instantly.
Sealing of the straw is performed after vitrification which makes it easier for the operator to perform the critical dehydration steps of vitrification within the important time frames. When the embryos are to be warmed, the RapidStraw is cut and the Rapid-i retrieved without any contact with surrounding materials, allowing for safe aseptic handling.
The Rapid-i Vitrification System is an integrated, convenient and closed system for vitrification.
The device, media and supporting accessories in the system are developed to function together.
To simplify identification and cryostorage management, the Rapid-i straws are available with five different colour codes; blue, green, red, yellow and transparent.
Get to know the benefits of working with Rapid-i vitrification system in this short movie.
RapidVit™ and RapidWarm™ media are used at 37˚C to maintain physiological temperature during dehydration and re-hydration procedures. Read a blog post on why 37⁰C is the optimal temperature for vitrification.
Loading the Rapid-i is as simple as it is smart. By holding embryos with surface tension in a defined and minute volume, vitrification in super-cooled air occurs instantly without contact with liquid nitrogen. During warming, oocytes and embryos come off easily into the warming solution. We have collected our best tips & tricks for successful vitrification in a blog post.
Several publications and trials have shown that the closed system Rapid-i provides successful outcomes after vitrification.
1. Hashimoto, S. Et al.; J Assist Reprod Genet. (2013): 30(3): 371-6
Watch a movie with one of our embryologists demonstrating how to use the Rapid-i Vitrification System in a clinical setting.
Mastering the slow cooling rates and controlling the concentration of cryoprotectants is critical for initiation of ice nucleation and successful freezing.
With the introduction of FreezeKit Cleave™ and ThawKit Cleave™ Vitrolife offers a procedure for slow freezing based on today’s knowledge of cell physiology and cryopreservation.
FreezeKit Cleave and ThawKit Cleave make the whole slow freezing procedure faster and more convenient. With fewer steps compared to old protocols they save time, reduce operator variability and simplify the training process.
Most importantly, embryos are exposed to less stress with the fewer steps and shorter handling times.
A randomised comparison at two clinics in Sweden compared FreezeKit Cleave and ThawKit Cleave with an existing commercial product for freezing of cleavage-stage embryos. The primary endpoint was blastomere survival rate. A statistically significant difference in blastomere survival was seen between the two methods.
2. Vitrolife: Data on file.
The 5-well dish provides rapid temperate equilibration, uniform across the whole dish. The ramped edges in the wells allows for quick localisation of the embryos in all cryo preservation procedures.
Watch the movie on how to cryopreserve cleavage stage embryos using Vitrolife products and the slow freezing method.
Read guest blogger professor David Edgar’s blog post about slow freezing and its role in cryopreservation
In this presentation Dr. Edgar explains why slow freezing still has a role in cryo preservation even if vitrification is becoming increasingly more common.