G-2™ PLUS and G-2™ provides the embryo with a wide range of amino acids and hyaluronan for optimised blastocyst development.
Description G-2 PLUS and G-2 are bicarbonate buffered media containing hyaluronan. G-2 PLUS also contains human serum albumin.
Purpose Medium for culture of embryos from day 3 to the blastocyst stage.
Storage Store dark at +2 to +8°C
G-2 PLUS and G-2 are two different media for culture of human embryos from the 8-cell stage on day three until the blastocyst stage. G-2 PLUS is supplemented with human serum albumin, HSA, and is ready to use. G-2 must be supplemented with G-MM™ or HSA-solution™.
Both media contains amino acids to support embryo viability and hyaluronan to improve development and cryosurvivability and to facilitate implantation. They also contain vitamins that function as antioxidants to protect the embryo against reactive oxygen species during culture in vitro.
Each product in the G-Series™ is developed to resemble conditions in the female reproductive tract and fulfil embryo requirements.
Ensure optimal conditions before, during and after culture by using the whole G-Series. All G-Series media share the same basic composition to secure viability and implantation potential. Osmolality, pH and supporting compounds are all kept constant. This foundation prevents intracellular stresses as the embryos progress through the IVF process.
Manual G-Series. For US
This manual describes how to use all media in the G-Series: G-RINSE, G-MOPS, G-GAMETE, G-IVF, G-1, G-2, G-TL, G-PGD, HSA-solution, G-MM, ICSI, HYASEx-10x, OVOIL, SpermGrad and EmbryoGlue
Short protocol - Embryo culture
This short protocol describes how to culture embryos from after fertilisation until the blastocyst stage using the G-Series™ sequential media G-1™ PLUS, G-2™ PLUS and OVOIL™.
Short protocol - Embryo Culture using sequential media and Primo Vision™ time-lapse embryo monitoring system – Method 1
This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for handling of oocytes and embryo culture in Primo Vision™ time-lapse monitoring system and change medium on day 3 by preparing a new dish.
Short protocol - Embryo Culture using sequential media and Primo Vision™ time-lapse embryo monitoring system – Method 2
This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS™ PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for oocyte handling and embryo culture in Primo Vision time-lapse monitoring system and change medium on day 3 using the same dish.
Short protocol - Blastocyst trophectoderm biopsy
This short protocol describes how to perform blastocyst trophectoderm biopsy using OVOIL™ and G-Series™ media, G-2™ PLUS or G-TL™ and G-MOPS™ PLUS
G-Series culture media shown to increase blastocyst development compared to control
In a study by Matsuoka et al, embryo culture was done either Vitrolife’s G-Series™ culture media or a mix of sequential culture media (control). The results show that good-quality blastocyst development was more frequent after culture in G-Series media containing hyaluronan.
Optimised clinical outcomes with blastocyst culture and cryopreservation using Vitrolife G-Series culture media
A study by Zhu et al demonstrate that extended culture before cryopreservation allows for selection of viable embryos with a high chance of implantation after warming. The high blastocyst formation rate (73.8%) using Vitrolife culture media, together with an efficient cryopreservation program resulting in survival rates of >96% should eliminate any earlier concerns about blastocyst culture and cryopreservation.
Embryo culture in G-Series results in significantly higher cell numbers and implantation rates compared to culture in HTF
A study by Kleijkers show that besides cell numbers and implantations rates, gene expression and certain pathways in preimplantation embryos are also affected by culture medium.
Safety data sheets (SDS)
Safety data sheet for G-2
Safety data sheets (SDS)
SDS G-2 PLUS
Safety data sheet for G-2 PLUS