For rinsing of contact materials and washing of the cervix before oocyte aspiration and embryo transfer.
Description Bicarbonate buffered salt solution. Not for culture.
Purpose Solution for rinsing of contact materials and for washing of the cervix.
Application For use after pre-equilibration at +37ºC and 6 % CO2.
Storage Store dark at +2 to +8°C
Use G-RINSE to test and rinse oocyte retrieval needles, wash the cervix prior to retrieval and transfer and for rinsing of contact materials. G-RINSE contains gentamicin, salts and a carbohydrate with the same osmolality as the other G-Series™ media. Rinsing with G-RINSE ensures that no dilution affects your culture.
It is well known that the manufacturing process of traditional non IVF certified plastics such as dishes and test tubes may leave debris behind in the products. This debris can easily be observed floating in the dishes and tubes after adding media. These particles will adhere to the zonas of embryos when culturing or when rinsing through these disposables. This may affect the development of the embryos.
The viability of embryos is strongly correlated to cell numbers1. To confirm that rinsing of dishes improves embryo viability, the cell number of blastocysts were counted after culture in rinsed and non rinsed dishes.
In order to minimise the amount of debris present in plasticware, Vitrolife recommends pre-rinsing all contact materials before use whenever possible. The exception would be Vitrolife Labware products, uniquely certified for human IVF.
Ensure optimal conditions before, during and after culture by using the whole G-Series. All G-Series media share the same basic composition to secure viability and implantation potential. Osmolality, pH and supporting compounds are all kept constant. This foundation prevents intracellular stresses as the embryos progress through the IVF process.
Manual G-Series. For US
This manual describes how to use all media in the G-Series: G-RINSE, G-MOPS, G-GAMETE, G-IVF, G-1, G-2, G-TL, G-PGD, HSA-solution, G-MM, ICSI, HYASEx-10x, OVOIL, SpermGrad and EmbryoGlue
Short protocols - Oocyte retrieval and fertilisation assessment
This short protocol describes handling of oocytes and embryos in ambient atmosphere using G-Series™ media G-RINSE™, G-MOPS™, G-MOPS™ PLUS and G-IVF™ PLUS