OVOIL™

OVOIL is a 100% paraffin oil. OVOIL HEAVY is a synthetically high viscosity oil. Both of them are produced in a highly controlled process from extensively tested and selected LOTs of raw material.

Description OVOIL is a light paraffin oil. OVOIL HEAVY is a high viscosity oil. Both are sterile filtered.

Purpose Oil for covering of medium during in vitro fertilisation and micromanipulation procedures.

Application For use after equilibration at +37°C in CO2-incubator or ambient atmosphere, depending on the intended use.

Storage Store dark at +2 to +8°C. During transportation temperature may exceed +8°C but not +25°C for a maximum of seven days.

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10029 OVOIL™ - culture oil
1 x 100 mL
10174 OVOIL HEAVY™
1x100 mL

The choice is yours

While OVOIL™ is a light paraffin oil, OVOIL HEAVY™ is a synthetic paraffin oil – with a higher viscosity that some users feel offers improved handling qualities over lighter oils. While this naturally is a question of preference, we are pleased to now be able to offer two culture oils that cater to whichever yours might be.

OVOIL™ and OVOIL HEAVY™ have gone through the same rigorous quality testing,
making both oils ideally suited for the IVF procedures of today. This means you can
choose the oil that best corresponds with your handling preferences – without having
to compromise on quality and performance.

Together. all the way™

THROUGH THICK AND THIN

Oil for IVF should do nothing but protect the culture. It should not have any negative influence on the gametes, embryos or media. Most clinics today use oil to cover embryo culture dishes in order to maintain a stable temperature, osmolality and pH. The quality of such oil plays a very important role in IVF success.

Do you know the characteristics of the perfect oil for IVF?

Read this blog post for a guide through different types of oil and things to consider when choosing oil for your IVF culture.

Read blog post

The importance of peroxide-free oil for embryo culture

Download a scientific summary from Japanese researcher Otsuki to learn more about why high peroxide levels in oil can harm the culture system and the results from peroxide values in oil from different manufacturers.

Download summary

Comparison between mineral oil and paraffin oil

In a study by Tae et al. bovine embryo development was studied in cultures overlaid with either washed mineral or sterile paraffin oil.

Sterile filtered paraffin oil overlay resulted in significantly higher (p<0.05) development rate to morula (44.8% versus 30.6%) and blastocyst (32.8% versus 21.7%) than washed mineral oil.

REF: Tae, J.C. et al. J. Assist Reprod & Gen. 2006

Ovoil Heavy™

OVOIL HEAVY™ is not just any other oil.

OVOIL HEAVY is the world’s first synthetically derived paraffin oil – developed, manufactured, tested and certified specifically for clinical IVF. With consistently high lot-to-lot quality and increased viscosity, OVOIL HEAVY™ provides exceptional safety for your embryo culture while improving handling qualities in the lab.

Read blog post

Improved handling thanks to higher viscosity

It is important to have an oil that is easy to handle in the laboratory. One of the main benefits of OVOIL HEAVY™ is its higher viscosity, which in turn provides significantly improved handling qualities in the lab. 83,3% prefer OVOIL HEAVY when compared with their standard culture oil.

Light mineral oil in the left dish, OVOIL HEAVY in the right. Note how the droplets are less affected and you don't get oil as close to the rim in the right dish.

Refence: Embryotools data on file 2021

Extensive quality testing

OVOIL HEAVY™ is an extensively quality tested product:

  • Human sperm oil assay
  • 1-cell mouse embryo assays
  • Sensitive CF1 mouse strain on raw material
  • 1 cell, F1 hybrid mouse on finished product
  • Mouse embryo assays with multiple endpoints for
    grading development
  • Blastocyst differentiation staining and cell counts
  • Micro droplet oil test

Let OVOIL HEAVY protect your embryo culture

Download the research summary for OVOIL HEAVY and learn why it provides exceptional safety for your embryo culture while improving handling qualities in the lab.

Download research summary

It’s not what you do, it’s the way that you do it!

Download the scientific study abstract by Pa H. Vang, which show that the blastocyst cell count performed on MEA tests, are more sensitive endpoint than embryo development. End users should consider how a MEA is performed. It will help to minimize the risk of using suboptimal components within an IVF cycle, which will maximize the chance of success for each patient.

Download the MEA abstract

When comparing Ovoil Heavy to former oils I felt it offers me as an embryologist many handling benefits. I like the viscosity, how easy it is to pipette and there is much less spilling when using it. The higher viscosity allows easier control when injecting sperm during ICSI. I trust this oil knowing that it is heavily quality tested with specific MEA for oil. Having visited the Vitrolife lab and observing the process of quality control that were used to manufacture their products I can 100% confidently recommend their heavy oil.

- Van Pham, Chief Embryologist at Aspire Fertility in Houston, The Prelude Network

We have been given the opportunity to test Ovoil Heavy at the American Hospital of Paris. Happy users of the regular OVOIL for years we could not imagine to find a higher quality product. In fact Ovoil Heavy happened to be a more easy to use product. It's high viscosity helps avoiding splashing and spilling during dish preparation and handling still with the same premium quality. Ovoil Heavy met all our expectations and we decided to switch from the regular Ovoil to the heavy version.

- Dr. Frida Entezami, Lab manager of American Hospital of Paris

Ovoil™

OVOIL is not just oil

Oils from six different manufacturers were tested and embryo development and viability was evaluated.

OVOIL showed significantly better development (p<0.01) for all embryo viability parameters studied, number of cells in the trophectoderm and in the inner cell mass, and total number of cells in the blastocysts.

REF: D. Linck, SIRT, Australia. 2008.

OVOIL - extensively quality tested

  • Sperm sensitivity testing
  • 1-cell mouse embryo assays
    • Sensitive CF1 mouse strain on raw material
    • 1 cell, F1 hybrid mouse on finished product
    • Mouse embryo assays with multiple endpoints
    • Blastocyst differentiation staining and cell counts

Read more on how to ensure embryo viablity in your culture system:

Read blog post

Optimise all the way with G-Series

Ensure optimal conditions before, during and after culture by using the whole G-Series™. All G-Series media share the same basic composition to secure viability and implantation potential. Osmolality, pH and supporting compounds are all kept constant. This foundation prevents intracellular stresses as the embryos progress through the IVF process.

Support Documents

Documents Language

Package inserts
OVOIL™

Package inserts

English

Package insert OVOIL HEAVY

Package inserts
OVOIL™

Package inserts

English

Package insert OVOIL

Short protocols
OVOIL™

Short protocols

English

Short protocol - Embryo culture

This short protocol describes how to culture embryos from after fertilisation until the blastocyst stage using the G-Series™ sequential media G-1™ PLUS, G-2™ PLUS and OVOIL™.

Short protocols
OVOIL™

Short protocols

English

Short protocols - ICSI

This short protocol describes how to perform ICSI using ICSI™, HYASE™-10x, OVOIL™ and G-Series medium G-MOPS™ PLUS

Short protocols
OVOIL™

Short protocols

English

Short protocol - Embryo Culture using sequential media and Primo Vision™ time-lapse embryo monitoring system – Method 1

This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for handling of oocytes and embryo culture in Primo Vision™ time-lapse monitoring system and change medium on day 3 by preparing a new dish.

Short protocols
OVOIL™

Short protocols

English

Short protocol - Embryo Culture using sequential media and Primo Vision™ time-lapse embryo monitoring system – Method 2

This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS™ PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for oocyte handling and embryo culture in Primo Vision time-lapse monitoring system and change medium on day 3 using the same dish.

Short protocols
OVOIL™

Short protocols

English

Short protocol - Embryo Culture using G-TL™ with Primo Vision™ Time-lapse monitoring system

This short protocol describes handling of embryos for culture in Primo Vision time-lapse system using G-Series ™media G-MOPS™, G-MOPS™ PLUS, OVOIL™ and G-TL™.

Short protocols
OVOIL™

Short protocols

English

Short protocol - Blastocyst trophectoderm biopsy

This short protocol describes how to perform blastocyst trophectoderm biopsy using OVOIL™ and G-Series™ media, G-2™ PLUS or G-TL™ and G-MOPS™ PLUS

Scientific summaries
OVOIL™

Scientific summaries

English

Comparison of cover oils - OVOIL gives both more good and top quality embryos

In a study by Sifer et el, microdroplet embryo cultures covered with OVOIL from Vitrolife gave a significantly increased mean number of good and top quality embryos at day 3, compared to other commercial available oils. This difference may be explained by the origin of raw materials and the difference in quality tests performed by each company.

Product manuals
OVOIL™

Product manuals

English

Manual G-Series. International

This manual describes how to use all media in the G-Series: G-RINSE, G-MOPS, G-GAMETE, G-IVF, G-1, G-2, G-TL, G-PGD, HSA-solution, G-MM, ICSI, HYASEx-10x, OVOIL , SpermGrad and EmbryoGlue.

Product manuals
OVOIL™

Product manuals

English

Manual G-Series. For US

This manual describes how to use all media in the G-Series: G-RINSE, G-MOPS, G-GAMETE, G-IVF, G-1, G-2, G-TL, G-PGD, HSA-solution, G-MM, ICSI, HYASEx-10x, OVOIL, SpermGrad and EmbryoGlue

Safety data sheets (SDS)
OVOIL™

Safety data sheets (SDS)

English

SDS OVOIL HEAVY

Safety data sheet for OVOIL

Safety data sheets (SDS)
OVOIL™

Safety data sheets (SDS)

English

SDS OVOIL

Safety data sheet for OVOIL

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