Gx Media
Gx-MOPS™ PLUS, Gx-IVF™ and Gx-TL™ with a triple antioxidant combination1,2 is the latest generation of Vitrolife media. The triple antioxidant protection for gamete and embryo handling, fertilisation and culture have a highly beneficial effect on embryo development when used individually and to a greater effect when in combination.
Storage Store dark at +2 to +8ºC.
Gx Media – Triple antioxidant protection for gametes and embryos
Reactive oxygen species (ROS) are present throughout the IVF cycle and pose a significant threat to embryo culture. To protect embryos, we developed Gx Media – with a unique triple antioxidant protection that ensures embryo safety throughout the entire IVF cycle.
Triple antioxidants working together
Three antioxidants have been added to our gamete-handling, fertilisation, and culture media to form a protective barrier against oxidative stress.
Acetyl-L-Carnitine improves oocyte chromosomal structure and blastocyst development while reducing apoptosis.1
Alpha-Lipoic Acid significantly improves embryo development and cell numbers at 20% oxygen.2
N-Acetyl-L-Cysteine increases glutathione (GSH) levels, cleavage and blastocyst rates. 3
Pioneering an antioxidant system
Oxidative stress is an inevitable outcome of metabolism and in vitro culture. Our research led to Gx Media, an antioxidant system that mitigates oxidative stress in gametes and embryos in both ambient and reduced oxygen conditions.4, 5, 6 Gx-MOPS™ PLUS, Gx-IVF™ and Gx-TL™ with this triple antioxidant combination7, 8 is the latest generation of Vitrolife media.
Maximum impact when used in combination for fertilisation and culture
The graph compares Control, no antioxidants (AOX), to AOX use during fertilisation and/or culture, showing the greatest effect when used for both.8
Fertility success
Launched in 2018, Gx-MOPS PLUS, Gx-IVF and Gx-TL with a triple antioxidant combination7, 8 have been compared in multiple randomised controlled trials with standard G-series media (G-MOPS PLUS, G-IVF PLUS and G-TL/G-1 PLUS/ G-2 PLUS)9 - 13, showing similar trends.
Improved ICSI fertilisation
Gx Media significantly improved ICSI fertilisation rates when used for sperm preparation, oocyte collection and subsequent ICSI fertilisation.10, 12-14
Higher pregnancy rates for women 35-40 years
Gx Media or antioxidant-enhanced G-series media significantly improved pregnancy rates in frozen embryo transfers when used continiously throughout the IVF process9, 12
Improved fertilisation rate leads to more usable blastocysts
“Addition of antioxidants to media during gamete collection and ICSI can increase fertilisation rate and reduce the frequency of failed fertilisation cycles. This results in more blastocysts available for transfer and cryopreservation per egg collection, which potentially could result in higher cumulative pregnancy rates. There was no effect of antioxidants on blastocyst development or pregnancy rates from fresh single embryo transfers.”
Improved ongoing pregnancy rate per frozen embryo transfer
“With regards to transfer outcomes, it was determined that the presence of antioxidants conferred the greatest benefit to patients 35–40 years, with a significant increase in clinical pregnancy rate and ongoing pregnancy rate.”
Live birth per single vitrified blastocyst transfer
“We found a marked improvement in the clinical outcomes of patients aged 35-40 years old by using the culture media supplemented with AOX throughout of the IVF process, from gamete handling and preparation, insemination, and the subsequent blastocyst culture.”
Antioxidant trio enhances embryo viability and IVF outcomes
The three antioxidants we have selected have a highly beneficial effect on embryo development when used individually and to a greater effect when in combination.
Acetyl-L-Carnitine, Alpha-Lipoic Acid and N-Acetyl-L-Cysteine have a highly beneficial effect on embryo development when used in combination.7, 8
Improved embryo viability in ageing gametes.9, 15
Benefits are greatest at 20% oxygen but are also present at 5% oxygen.7
The three antioxidants should be present in gamete-handling media, IVF media and culture media.7, 8, 9, 15
Improved ICSI fertilisation rates when used for sperm preparation, oocyte collection and subsequent ICSI fertilisation.9, 12-14
Significantly improved pregnancy rates for women aged 35–40 years when used continiously throughout the IVF process.9, 12, 13
David K. Gardner about antioxidants in culture media moving into the future
Consistent with our holistic approach of growing embryos, optimising the culture media and the environment using time-lapse incubation, I see that the inclusion of the triple antioxidants (or Gx Media) from gamete collection all the way to embryo transfer is a very important component to reducing stress and maintain the viability of our embryos in the laboratory.
- Prof. David K. Gardner at School of BioSciences and Scientific Director at Melbourne IVF
Oxidative stress - effect on embryo development
Gametes and embryos are highly sensitive to oxidative stress. When outside the body, they are deprived of the natural detoxification system. At the same time, manipulations during IVF correlate with numerous external risks such as: unphysiologically high oxygen concentration during the entire culture period or during some procedures (high oxygen is detrimental by itself); reactive oxygen species (ROS), which are produced as a byproduct of aerobic cellular metabolism; quality of plasticware and oil overlay, which may provoke an increase in ROS synthesis; shear stress, possible temperature fluctuations and light exposure. These factors can decrease developmental capacity and viability of resulting embryos. Importantly, once a gamete or embryo has been exposed to a specific stressor, it is common for them to be more sensitive to any additional burden, highlighting a paramount importance of complex stress protection during IVF treatment.
"Changing our culture media was a critical decision for our lab’s success in improving blastocyst development rates. Vitrolife and the Gx Media intrigued us because of their innovative use of antioxidants to combat reactive oxygen species.
After conducting a trial showing an improvement in blastocyst development, we confidently implemented the culture system for all our patients. We've witnessed a significant increase in the formation of high-quality blastocysts.
I recommend Gx Media to my colleagues in the field. This shift to Gx Media has not only enhanced our blastocyst development but has also positively impacted the overall quality of care we provide to our patients."
- Khalied Kaskar, Laboratory Director, Austin, San Antonio
Webinar: 'Antioxidants in human IVF - An innovation in culture media'
In this webinar Professor David Gardner is discussing the importance of antioxidants as an element of ART media and the potential for improved embryo viability. He is sharing his experience leading to the development of culture media complemented with a unique mix of antioxidants, additionally discussing the results from current clinical studies.
This live webinar was held in August 2021.
Watch webinar
Extensively quality tested
Gx Media have gone through the same rigorous quality testing as all our other Vitrolife products. The mouse embryo assay (MEA) is the most valuable tool Vitrolife has to ensure safe and consistent products. Read more about how we perform MEA.
Read more
References
- Abdelrazik, H., A (2007) Fert Steril. 88, Supplement 1(0): S317.
- Linck, Larman and Gardner (2007) Fert Steril. 88: S36-S37.
- Furnus, C. C. et al. (2008) Anim Reprod Sci 109(1-4): 88-99.
- Gardner & Truong (2017). Hum Reprod., P-248, Article.
- Truong et al. (2022). Reproductive BioMedicine Online, 44(3), 393-410.
- Truong et al. (2016). Hum Reprod., 31(7), 1445-1454, Article G-MOPS PLUS, Ovoil, EmbryoScope, G-1 (v5), G-2 (v5) (animal data).
- Truong et al. Hum Reprod. Vol. 31. No.7 pp.1445- 1454, 2016.
- Truong et al. Hum Reprod. Vol. 32. No.12 pp. 2404-2413, 2017.
- Gardner DK. et al. Reprod Biomed Online. 2020;40(5):637-44.
- Kelley R. et al. Hum Reprod. 2023;38(Suppl. 1):O-235.
- Hardarson T. et al. Fert Steril. 2018;110(4):e51, O-115.
- Mizumoto S. et al. 2025 Culture media with antioxidants improved preimplantation embryo development and clinical outcomes of patients of advanced age. RBMO, Vol. 50, Iss 1, 104415.
- Kelley et al. 2025, A prospective randomised controlled trial of antioxidants in human IVF and embryo culture.
- Phang et al. EP-03, RBMO Volume 48 Issue S1 2024.
- Mizumoto et al., P221 ESHRE 2021.
Support Documents
Documents Language
Package inserts
English
Package insert Gx-MOPS PLUS - Multi-language
Document and Ref: REF 26903 version 05
Package inserts
English
Package insert Gx-IVF - Multi-language
Document and Ref: REF 26901 version 05
Package inserts
English
Package insert GxTL - Multi-language
Document and Ref: REF 26902, version 05
Safety data sheets (SDS)
English
SDS Gx-IVF
Document and Ref: Date Revised : 01/08/2025. Revision No : 4. Safety data sheet for Gx-IVF
Safety data sheets (SDS)
English
SDS Gx-MOPS PLUS
Document and Ref: Date Revised : 06/27/2023. Revision No : 2. Safety data sheet for Gx-MOPS PLUS
Safety data sheets (SDS)
English
SDS Gx-TL
Document and Ref: Date Revised : 06/27/2023. Revision No : 2. Safety data sheet for Gx-TL
SSCP
English
SSCP Gx-TL
Document and Ref: REP-2364. Version 5.0. 2025/03/10
SSCP
English
SSCP Gx-MOPS PLUS
Document and Ref: REP-2367. Version 4.0. 2025/04/10
SSCP
English
SSCP Gx-IVF
Document and Ref: REP-2361. Version 5.0. 2025/04/10
Short protocols
English
Gx-Series™ Blastocyst trophectoderm biopsy
Document and Ref: REF 19180. Version 1. Date of issue 220923. his short protocol describes how to perform Blastocyst trophectoderm biopsy with OVOIL™, Gx-MOPS™ PLUS and Gx-TL™
Short protocols
English
Gx-Series™ Gx-TL Embryo Culture
Document and Ref: REF 19181. Version 1. Date of issue 220923. This short protocol describes how to perform embryo culture using Gx-TL™, OVOIL™ and Gx-MOPS™ PLUS.
Short protocols
English
Gx-Series™ ICSI procedure
Document and Ref: REF 19179. Version 1. Date of issue 220923 This short protocol describes how to perform ICSI using ICSI™, HYASE™-10x, OVOIL™ and Gx-Series medium Gx-MOPS™ PLUS and Gx-TL™
Short protocols
English
Gx-Series™ Sperm preparation - Density gradient centrifugation method
Document and Ref: REF 19177. Version 1. Date of issue 220923. This short protocol describes how to perform sperm preparation with the density gradient method and fertilization using products Gx-Series™ medium Gx-IVF™ PLUS or SpermGrad RTU and SpermGrad™
Short protocols
English
Gx-Series™ Sperm preparation - Swim up method
Document and Ref: REF 19176. Version 1. Date of issue 220923. This short protocol describes how to perform sperm preparation with the swim-up method and fertilization of oocytes using Gx-series™ medium Gx-IVF™
Short protocols
English
Gx-Series™ Oocyte retrieval and fertilisation
Document and Ref: REF 19178. Version 1. Date of issue 220923. This short protocol describes handling of oocytes and embryos in ambient atmosphere using Gx-Series™ media G-RINSE™, G-MOPS™, Gx-MOPS™ PLUS and Gx-IVF™ PLUS
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